Improved Detection of Acidovorax Citrulli in Watermelon and Melon Seed
نویسندگان
چکیده
1 Institute of Plant Protection, Chinese Academy of Agriculture Science, Beijing, 100094, China 2 Department of Plant Pathology, China Agricultural University, Beijing, 100193, China 3 Current address: Technical Centre of PAIQ, Shenzhen Entry-Exit Inspection & Quarantine Bureau, Guangdong 518001, P.R. China 4 USDA/ARS, Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, 21702, USA 5 California Seed and Plant Laboratory, Elverta, CA 95626 USA (E-mail: [email protected])
منابع مشابه
Multilocus sequence typing reveals two evolutionary lineages of Acidovorax avenae subsp. citrulli.
Acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, has caused considerable damage to the watermelon and melon industry in China and the United States. Understanding the emergence and spread of this pathogen is important for controlling the disease. To build a fingerprinting database for reliable identification and tracking of strains of A. avenae subsp. citrulli, a multi...
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In recent years, use of the DNA-intercalating dye propidium monoazide (PMA) in real-time PCR has been reported as a novel method to detect viable bacteria in different types of samples, such as food, environmental, and microbiological samples. In this study, viable cells of Acidovorax citrulli, the causal agent of bacterial seedling blight and fruit blotch, were selectively detected and differe...
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To improve the simultaneous detection of two pathogens in cucurbit seed, a combination of magnetic capture hybridization (MCH) and multiplex real-time polymerase chain reaction (PCR) was developed. Single-stranded DNA hybridization capture probes targeting DNA of Acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, and Didymella bryoniae, causal agent of gummy stem blight,...
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Acidovorax citrulli is a seedborne bacterium that causes bacterial fruit blotch of cucurbit plants including watermelon and melon. A. citrulli strains can be divided into two major groups based on DNA fingerprint analyses and biochemical properties. Group I strains have been generally isolated from non-watermelon cucurbits, while group II strains are closely associated with watermelon. In the p...
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Acidovorax avenae subsp. citrulli is the causal agent of bacterial fruit blotch (BFB), a threatening disease of watermelon, melon, and other cucurbits. Despite the economic importance of BFB, relatively little is known about basic aspects of the pathogen's biology and the molecular basis of its interaction with host plants. To identify A. avenae subsp. citrulli genes associated with pathogenici...
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